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. 2005 Apr;25(8):3261–3275. doi: 10.1128/MCB.25.8.3261-3275.2005

FIG. 7.

FIG. 7.

Identification of MEF-2 family nuclear factors that interact with the AβH-J-J promoter 2. (A and B) Band shift assays were performed using J-MEF-2mer probe (−84 to −61) or mutant probe (mp, J-MEF-2Mmer) (Fig. 7A, lane 8) (Table 1) and 2 μg of nuclear extracts (NE) from RD (A) or C2C12-GM and -DM (B) cells, cultured in growth and differentiation medium, respectively. (-), probes were incubated with nuclear extracts in the absence of competing oligonucleotides. Competing oligonucleotides (Table 1) were added at a 100-fold molar excess with the exception of lanes 3 and 5 (50-fold molar excess). (C and D) Band shift and supershift assays were performed using J-MEF-2mer probe and 2 μg of NE from C2C12-DM or 8 μg of NE from soleus muscle. (-), control samples in the absence of competing oligonucleotides or antibodies; probe was incubated with nuclear extracts in the presence of the indicated competing oligonucleotides (at a 100-fold molar excess) or in the presence of antibodies (Ab) against MEF-2 family members (1 to 2 μg) or against myogenin (2 μg). In this figure, arrows indicate the specific complexes; arrowheads indicate complexes supershifted by antibody.