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. 2005 Apr;25(8):3261–3275. doi: 10.1128/MCB.25.8.3261-3275.2005

FIG. 8.

FIG. 8.

Transcriptional effect of mutation in the MEF-2 binding site of the AβH-J-J promoter 2. (A) C2C12-GM cells were transfected with wild-type (−159 and −48) and MEF-2 binding site mutant (−159 MEF-2 box mut) AβH-J-J P2 promoter reporter constructs (represented in the left side of the figure). Transient transfection and luciferase assays were performed in triplicate, and the data were normalized to Renilla luciferase activity and are reported as ratios (means ± SD) to the pGL3-basic firefly luciferase (LUC) reporter vector. (B) The same constructs presented in panel A were transiently transfected in rat soleus muscles as described for the experiment whose results are presented in Fig. 4. (C and D) The wild-type and mutant −159 constructs whose results are presented in panels A and B were utilized in the absence (black bars) or in the presence (white bars) of MEF-2A expression vector for C2C12-GM (C) and HeLa (D) cells transfections.