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. 2023 Dec 4;14:8016. doi: 10.1038/s41467-023-43507-5

Fig. 1. The BioTAC system enables rapid, accurate small molecule target-ID.

Fig. 1

A Schematic depicting the components of the BioTAC system. B Example molecule, Cpd 1, annotated with key functional groups. C Immunoblot analysis of BRD4 enrichment following treatment of HEK293 cells transiently transfected with miniTurboFKBP12F36V with the indicated compounds and 100 µM biotin at the 30 min timepoint (input = sample processing control, n = 3 biological replicates, Supplementary Fig. 1I, J). D Scatterplot displaying relative FC of streptavidin-enriched protein abundance following treatment of HEK293 cells transiently transfected with miniTurboFKBP12F36V with either DMSO or 1 µM Cpd 1 plus the indicated concentration of (+)-JQ1 competitor and 100 µM biotin at the 30 min timepoint. Only proteins with P-value < 0.05 (two-sample moderated T test) in both conditions depicted. FC fold-change, complete datasets in Supplementary Data 1, plotted individually in Supplementary Fig. 4. Source data are provided as a Source Data file.