Fig. 4. The BioTAC system is an extensible strategy for live-cell target-ID.
A Chemical Structure of Cpd 3. B Immunoblot analysis of Aurora A enrichment following treatment of HEK293 cells transiently transfected with miniTurboFKBP12F36V with the indicated compounds and 100 µM biotin at the 4 h timepoint (input = sample processing control, n = 2 biological replicates. C, D Proteomics using data-independent acquisition methods of streptavidin-enriched biotinylated proteins isolated from HEK293 cells transiently transfected with miniTurboFKBP12F36V and treated with DMSO, 1 µM Cpd 3, plus the indicated compounds and 100 µM biotin for 4 h, demonstrating enrichment and competition of known direct targets and complexed proteins. Only proteins with P-value < 0.05 (two-sample moderated T test) in both conditions depicted. FC fold-change, complete datasets in Supplementary Data 1. Source data are provided as a Source Data file.