FIG. 3.

(A) Overexpression of TCTP elevated Mcl-1 protein levels. Control (clones V-13 and V-14) or TCTP overexpressing Ba/F3hMcl-1 cells (clones T-20 and T-23) under normal growth conditions were lysed, and cell lysates were analyzed by immunoblotting with anti-FLAG, anti-hMcl-1, or anti-mMcl-1 antibodies to detect the ectopically overexpressed proteins (hMcl-1 and FLAG-TCTP) or endogenous mMcl-1, respectively. The same blot was also probed with anti-α-tubulin antibody to monitor the amount of proteins loaded for each sample. (B) TCTP delays IL-3-removal-induced cell death. IL-3 was removed from the culture of the indicated cell clones for various times, and the numbers of viable cells at each time points were determined by trypan blue staining. The percent survival relative to those seeded is plotted against hours after IL-3 removal. The results shown are means (± the standard deviation) of three independent experiments with very similar results.