FIG. 8.

The antiapoptotic activity of the TCTP-binding defective mutant K257V is compromised. Ba/F3 stable clones overexpressing wild-type or mutant Mcl-1 (two independent clones of each) were examined for cell survival after IL-3 removal. Cells undergoing cell death was quantified by annexin V staining. The top panel shows the relative cell survival of various clones as indicated after IL-3 removal. The immunoblot on the bottom shows the protein levels of ectopically expressed wild type or mutant Mcl-1 (K257V or K260V).