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. 2005 Apr;25(8):3007–3018. doi: 10.1128/MCB.25.8.3007-3018.2005

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Copyright © 2005, American Society for Microbiology

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FIG. 3. — Association of Pex13 mutant proteins with the docking complex. The indicated oleic acid-induced strains containing a chromosomal protein A tag at the PEX14 locus were analyzed for the presence of Pex13 in the docking complex that had been purified with ProtA-Pex14. In particular, the UTL-7A-derived pex13Δ mutant strains harboring plasmids designed to express Pex13 (pAS62), Pex13_loop (pAS76), Pex13_SH3 (pAS61), and Pex13_loop+SH3 (pAS75) were analyzed. (Upper panels) Immunoblots loaded with aliquots of proteins solubilized with 1% digitonin from total membrane fractions and decorated with anti-Pex13 and Pex14 antibodies. (Lower panels) Immunoblots showing the abundance of Pex14 and Pex13 in eluates. Numbers below the bottom panel denote the ratios of the intensities of the Pex13 signals to the corresponding Pex14 signals. The calculated intensity of the wild-type sample was set at 100%. n.d., not determined.