FIG. 5.

Involvement of Pex5 in the association of Pex13 with the Pex14-containing docking complex. The docking complex was isolated with ProtA-Pex14 from oleic acid-induced cells lacking Pex5 and expressing the indicated mutant versions of Pex13. (Lower panels) The amount of Pex13 coeluting with the docking complex was determined by immunoblot analysis. Numbers below the bottom panel denote the ratios of the intensities of the Pex13 signals to the corresponding Pex14 signals. The calculated intensity of the sample derived from pex5Δ pex13Δ cells expressing wild-type Pex13 was set at 100%. n.d., not determined. (Upper panels) Amounts of Pex14 and Pex13 present in solubilized membrane protein fractions.