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Influence of mutant versions of Pex13 and of Pex5 on Pex14 targeting. The localization of Pex14 in oleic acid-induced pex13Δ and pex5Δ pex13Δ cells expressing wild-type or mutant versions of Pex13 was compared to that of HA-Pex11 by double-immunofluorescence microscopy. Detection was achieved with rabbit polyclonal antibodies against Pex14 and anti-HA monoclonal antibodies followed by Alexa Fluor 488-labeled anti-mouse and Alexa Fluor 594-labeled anti-rabbit secondary antibodies.
