FIG. 7.
Dependence of matrix protein import on Pex5 in the absence of direct Pex13-Pex14 interactions. (A) Oleic acid-induced pex5Δ pex13Δ cells expressing mutant versions of Pex13 in combination with HA-Pex11 were processed for double-immunofluorescence microscopy with rabbit antibodies against PTS2 protein Fox3 and anti-HA monoclonal antibodies. The secondary antibodies used were Alexa Fluor 488-labeled anti-mouse IgG and Alexa Fluor 594-labeled anti-rabbit IgG. pex5Δ cells were used as a control for intact PTS2 import. (B and C) The same cells but additionally expressing Pex5W204A were similarly inspected for the localization of Fox3 (B) and Pcs60 (C).