TABLE 1.
Plasmids used in this study
| Plasmid | Descriptiona | Reference or source |
|---|---|---|
| pKat61 | PEX19 in pPC86/SalI-SacI | 42 |
| pPC86-PEX14 | PEX14 in pPC86 | 2 |
| pWG14/6 | PEX14 AXXA in pPC86 | 19 |
| pWG13/15 | PEX13 (1-386) E320K | 19 |
| pKat31 | PEX13 (1-151) in pPC97/SmaI-SpeI | 42 |
| pKat33 | PEX13 (1-264) in pPC97/SmaI-SpeI | 42 |
| pKat129 | PEX13 (151-264) in pPC97/SmaI-SpeI | This study |
| pKat145 | PEX13 (173-258) in pPC97/SalI-NotI | 42 |
| pKat42 | PEX13 (280-386) in pPC97/SmaI-SpeI | 42 |
| pAS40 | PEX13 (173-258) L236A in pPC97 | This study |
| pAS41 | PEX13 (173-258) I237A in pPC97 | This study |
| pAS42 | PEX13 (173-258) F240A in pPC97 | This study |
| pAS43 | PEX13 (173-258) F243A in pPC97 | This study |
| pAS82 | PEX13 (173-258) L236A-I237A-F240A-F243A (loop) in pPC97 | This study |
| pQE31-PEX14 | His6-PEX14 | 45 |
| pGEX4T-2-PEX14 | GST-PEX14 in pGEX4T-2/BamHI-EcoRI | This study |
| pAS61 | PEX13prom-PEX13 E320K (SH3) in YCplac111 | This study |
| pAS62 | PEX13prom-PEX13 in YCplac111 | This study |
| pAS75 | PEX13prom-PEX13 loop + SH3 in YCplac111 | This study |
| pAS76 | PEX13prom-PEX13 loop in YCplac111 | This study |
| pKat113 | PEX13prom-PEX13 in pRSTERM | 45 |
| pAS53 | PEX13prom-PEX13 SH3 in pRSTERM | This study |
| pAS71 | PEX13prom-PEX13 loop in pRSTERM | This study |
| pAS73 | PEX13prom-PEX13 loop + SH3 in pRSTERM | This study |
| pRSPMP27tag | PEX11prom-PEX11-HA in pRS315 | 27 |
| pAS81 | PEX5prom-PEX5 W204A in pRS414 | This study |
a
Numbers in parentheses indicate amino acids. prom, promoter.