FIG.2.

dE2F2 colocalizes with dDP on polytene chromosomes. (A to F) Wild-type polytene chromosomes were stained with anti-dE2F2 and anti-dDP antibodies and counterstained with YOYO to visualize DNA. (G to I) Higher magnification of a fragment of wild-type polytene chromosome that was costained with anti-dE2F2 and anti-dDP antibodies. The arrows indicate one of the sites that stains for dDP but not for dE2F2. (J to L) dDP mutant chromosomes stained with anti-dE2F2 antibody. (M to O) de2f2 mutant chromosomes stained with anti-dDP antibody. Note the reduction in the number of dDP binding sites in the de2f2 mutant compared to the wild type. (P to R) de2f2 de2f1 double-mutant chromosomes stained with anti-dDP antibody.