FIG. 5.

dE2F1 is not sufficient to induce E2F-dependent transcription. (A and B) Eye disks were stained with anti-dE2F1 antibody. (A) In a wild-type disk, dE2F1 is expressed in the morphogenetic furrow and in the cells anterior and posterior to the furrow. (B) No staining with anti-dE2F1 antibody is observed in the eye disks trans-heterozygous for de2f1 mutant alleles. (C) In situ hybridization of eye disks from wild-type larvae with PCNA probe. PCNA transcripts are detected in cells posterior to the furrow. (D) Direct comparison of dE2F1 (red) and PCNA (green) expression patterns using a transgene producing a GFP-PCNA fusion protein. Arrow, position of the morphogenetic furrow.