FIG. 9.

Cell cycle characteristics of dDP mutant cells. Clones of dDP mutant cells were induced with ey-FLP, and eye imaginal disks were labeled with BrdU to visualize cells in S phase (A to D, J, and K) or stained with anti-cyclin A antibody (E and F), anti-cyclin B antibody (G and H), and anti-phos-H3 antibody (I to L). Mutant cells lack GFP signal. (A and B) Cells in dDP mutant clones incorporate BrdU at the same time as wild-type cells but with less efficiency. (C and D) Reduced efficiency of BrdU labeling in dDP mutant clones is fully rescued by a dDP genomic rescue construct. The timing of induction of cyclin A (E and F) and cyclin B (G and H) is normal in a dDP mutant clone. The number of cells in mitosis is reduced in dDP mutant cells, as visualized by anti-phos-H3 staining (I). Ectopic expression of cyclin E restores the efficiency of BrdU incorporation (J and K) but not the reduced number of cells entering mitosis (L) in dDP mutant clones. Arrow, position of the morphogenetic furrow.