. 2023 Nov 18;101:106697. doi: 10.1016/j.ultsonch.2023.106697

Table 2.

Effect of ultrasound on molecular weight distribution and the secondary structure.

	Percentage content (%)	Control	150 W 40 min	300 W 20 min	300 W 40 min	300 W 60 min	450 W 20 min	450 W 40 min
Molecular weights distribution	＜500 Da	45.25 ± 0.60^d	54.72 ± 0.07^a	47.49 ± 0.95^bc	46.99 ± 0.48^c	46.50 ± 0.51^cd	48.69 ± 1.09^b	45.11 ± 0.74^d
	＜1 kDa	58.99 ± 0.71^c	67.48 ± 0.18^a	61.46 ± 1.07^b	60.95 ± 0.34^b	60.67 ± 0.27^b	62.20 ± 1.31^b	58.39 ± 0.68^c
	＜3 kDa	76.04 ± 0.69^c	82.25 ± 0.41^a	78.57 ± 0.86^b	78.80 ± 0.24^b	78.11 ± 0.06^b	78.89 ± 1.23^b	76.09 ± 0.43^c
	＜8 kDa	87.89 ± 0.65^c	91.56 ± 0.51^a	90.01 ± 0.65^b	90.44 ± 0.27^ab	89.66 ± 0.29^b	89.91 ± 1.04^b	88.09 ± 0.44^c
Secondary structure	α-helix	29.33 ± 4.93^c	44.33 ± 1.15^b	44.00 ± 0.00^b	48.67 ± 1.15^a	51.33 ± 0.58^a	42.67 ± 0.58^b	52.33 ± 0.58^a
	β-chain	38.00 ± 7.81^a	34.67 ± 0.58^ab	34.00 ± 1.00^abc	30.67 ± 0.58^bc	28.67 ± 1.53^c	35.00 ± 0.00^ab	28.67 ± 1.53^c
	random coil	34.33 ± 7.50^a	21.33 ± 0.58^b	21.67 ± 0.58^b	20.33 ± 0.58^b	20.00 ± 0.00^b	22.00 ± 0.00^b	19.67 ± 0.58^b

Different letters (a-d) in the same row indicate significant differences between different ultrasound treatments (P < 0.05).