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. 2023 Nov 13;9(11):e22388. doi: 10.1016/j.heliyon.2023.e22388

Table 1.

Kinetic parameters for the wild-type CotA and Q441A using ABTS as substrate.

Laccase Km (mM)a kcat (s−1) kcat/Km (s−1 mM−1)
WT 0.079 ± 0.005 15.36 ± 2.41 194.09
Q441A 0.052 ± 0.003 20.48 ± 2.18 392.07
a

Laccase activity was calculated at 37°C with 100 mM sodium citrate buffer (pH 4.5). ABTS was chosen as substrate. Km and kcat were determined by Michaelis-Menten equation.