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. 2023 Nov 13;9(11):e22388. doi: 10.1016/j.heliyon.2023.e22388

Table 2.

Kinetic parameters for the wild-type CotA and Q441A using AFB1 as substrate.

Laccase Km (mM)a kcat (s−1) kcat/Km (s−1 mM−1)
WT 0.131 ± 0.004 0.054 ± 0.008 0.412
Q441A 0.084 ± 0.002 0.060 ± 0.005 0.714
a

Laccase activity was calculated at 37°C with 100 mM sodium phosphate buffer (pH 8.0). AFB1 was chosen as substrate. Km and kcat were determined by Michaelis-Menten equation.