. 2023 Nov 13;9(11):e22388. doi: 10.1016/j.heliyon.2023.e22388

Table 3.

Effect of metal ions on activity of wild-type CotA and Q441A using AFB₁ as substrate.

Metal ion (10 mM)	Residual activity (%)		SEM	P-value
Metal ion (10 mM)	WT	Q441A	SEM	P-value
–	100^a	100^a	–	–
Cu²⁺	78.31 ± 11.88^bc	86.19 ± 6.53^ab	9.581	0.457
Mg²⁺	88.29 ± 8.46^ab	102.20 ± 1.64^a	6.092	0.085
Ca²⁺	70.92 ± 7.41^bc	76.16 ± 6.16^bc	6.817	0.484
Co²⁺	69.89 ± 4.94^bc	68.06 ± 12.73^c	9.656	0.859
Zn²⁺	30.03 ± 2.30^Bd	40.99 ± 3.13^Ad	2.750	0.016
Mn²⁺	60.40 ± 2.73^c	61.12 ± 2.57^c	2.652	0.798
Na⁺	86.87 ± 1.70^Bab	97.13 ± 0.47^Aa	1.249	0.001
SEM	4.368	4.370
P-value	<0.0001	<0.0001

^{A, B} Different letters indicate significant different (P < 0.05) between WT and Q441A.

^a-d Different letters indicate significant different (P < 0.05) among various metal ions.