FIG. 7.

CpxP combats extracytoplasmic stress. Ten microliters of 40% maltose was added to filter discs that had been placed on lawns of strains WBS164 (MC4100 Φ(lamB-lacZX90) Hyb42-1[λp1(209)]) (lanes 1 and 2), SP9 (WBS164 degP::Tn10) (lanes 3 and 4), SP10 (WBS164 λplacMu53[cpxP-lacZ]) (lanes 5 and 6), and SP24 (WBS164 degP::Tn10 λplacMu53[cpxP-lacZ]) (lanes 7 and 8). Strains in odd-numbered lanes were transformed with pBR322 (control for pLD404); strains in even-numbered lanes were transformed with pLD404 (overproduces NlpE and activates the Cpx signal transduction pathway). The values displayed along the y axis (zone of clearing) represent the amount of growth inhibition caused by the addition of maltose. The zone of clearing value is defined as the diameter of growth inhibition around the maltose-saturated filter disc minus the diameter of the filter disc itself (7 mm). The maltose disc assays were performed on M63 minimal agar containing 50 μg of ampicillin per ml and 0.2% glycerol as a carbon source.