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. 2023 Nov 17;101:106698. doi: 10.1016/j.ultsonch.2023.106698

Fig. 1.

Fig. 1

Preparation of cholesterol-containing MRS medium. (A): Preparation of cholesterol solution. Figure a-d shows the cholesterol dissolved in anhydrous ethanol, control 1 (0.8 g cholesterol, 2 g bovine bile salt, 1 g sucrose fatty acid ester, 1 mLTween-80, 5 mL anhydrous ethanol, water bath at 80 °C for 10 min, after 80w sonication over 0.45 µm filter membrane), control 2 (ether: Tween-80: cholesterol of 8:1:1, v/v/w), and experimental group (ether: Tween-80: cholesterol of 7:2:1, v/v/w). (B): Cholesterol-containing MRS liquid medium. Figures a-c show the normal MRS liquid medium, MRS medium after adding cholesterol solution and homogenization, and MRS medium after adding cholesterol solution and resting for 7 d, respectively. (C): Cholesterol-containing MRS medium after 37 °C fermenting 16 h and 4 °C, 8000 × g centrifugating 10 min. Figure a-d shows the blank MRS medium, MRS medium after homogenization with cholesterol solution, MRS medium inoculated with 2 % (v/v) LAB containing cholesterol solution, and MRS medium after 7 d of resting, respectively.