TABLE 1.
Production of SefA, FimA, AgfA, and SefD fimbrins by S. enteritidis 3b and S. enteritidis 3b-122
| Mediuma | °C | rpm | Fimbrin productionb
|
|||||||
|---|---|---|---|---|---|---|---|---|---|---|
|
S. enteritidis 3b
|
S. enteritidis 3b-122
|
|||||||||
| SefA | FimA | AgfA | SefD | SefA | FimA | AgfA | SefD | |||
| TFB | 28 | 0 | − | + | − | +++ | − | − | − | +++ |
| TFB | 250 | − | ++ | ± | +++ | − | − | − | +++ | |
| TFB | 37 | 0 | + | ++ | − | +++ | − | − | − | +++ |
| TFB | 250 | −c | ++ | ± | +++ | − | − | + | +++ | |
| LB | 28 | 0 | − | ++ | ++ | +++ | − | − | − | +++ |
| LB | 250 | − | ++ | +++ | +++ | − | − | +++ | +++ | |
| LB | 37 | 0 | +++ | +++ | ++ | +++ | − | − | − | +++ |
| LB | 250 | ++ | ++ | +++ | +++ | − | − | + | +++ | |
| CFA | 28 | 0 | − | ++ | +++ | +++ | − | ± | +++ | +++ |
| CFA | 250 | − | + | +++ | +++ | − | ± | +++ | +++ | |
| CFA | 37 | 0 | +++ | ++ | +++ | +++ | − | ± | +++ | +++ |
| CFA | 250 | −c | + | +++ | +++ | − | ± | +++ | +++ | |
| T | 28 | 0 | − | ++ | +++ | +++ | − | − | +++ | +++ |
| T | 250 | − | + | +++ | +++ | − | − | +++ | +++ | |
| T | 37 | 0 | + | ++ | +++ | +++ | − | − | +++ | +++ |
| T | 250 | −c | + | +++ | +++ | − | − | +++ | +++ | |
a
All cultures were grown in liquid medium for 24 (TFB and LB) or 48 h (T and CFA).
b
The intensities of fimbrin bands detected on Western blots are indicated as follows: −, none detected; ±, very faint; +, weak; ++, moderate; +++, strong.
c
SefA was not detected on four of six Western blots but a very faint SefA band was detected on two of six Western blots.