Table 2.

Example calculation of mean absorbance change using raw data

Reading	Basal well 1	Basal well 2	Activated well 1	Activated well 2
Reading ‘22’ minus ‘21’	-0.0040	-0.0030	-0.0040	-0.0040
Reading ‘23’ minus ‘22’	-0.0030	-0.0040	-0.0030	-0.0030
Reading ‘24’ minus ‘23’	-0.0040	-0.0030	-0.0040	-0.0040
Reading ‘25’ minus ‘24’	-0.0040	-0.0030	-0.0040	-0.0040
Reading ‘26’ minus ‘25’	-0.0030	-0.0040	-0.0030	-0.0040
Reading ‘27’ minus ‘26’	-0.0030	-0.0030	-0.0040	-0.0030
Reading ‘28’ minus ‘27’	-0.0040	-0.0040	-0.0040	-0.0040
Reading ‘29’ minus ‘28’	-0.0040	-0.0030	-0.0030	-0.0040
Reading ‘30’ minus ‘29’	-0.0030	-0.0030	-0.0040	-0.0030
Reading ‘31’ minus ‘30’	-0.0030	-0.0040	-0.0040	-0.0040
Reading ‘32‘ minus ‘31’	-0.0040	-0.0030	-0.0030	-0.0030
Reading ‘33‘ minus ‘32’	-0.0030	-0.0030	-0.0040	-0.0040
Reading ’34‘ minus ‘33’	-0.0030	-0.0040	-0.0040	-0.0040
Reading ‘35‘ minus ‘34’	-0.0040	-0.0030	-0.0030	-0.0030
Reading ‘36‘ minus ‘35’	-0.0040	-0.0030	-0.0040	-0.0040
Reading ‘37‘ minus ‘36’	-0.0030	-0.0030	-0.0030	-0.0030
Reading ‘38‘ minus ‘37’	-0.0030	-0.0040	-0.0040	-0.0040
Reading ‘39’ minus ‘38’	-0.0030	-0.0030	-0.0030	-0.0030
Reading ‘40’ minus ‘39’	-0.0040	-0.0030	-0.0030	-0.0030
Mean absorbance change	-0.0035	-0.0033	-0.0036	-0.0036