Fig. 6.

A: FADS3 expression in HAP1 DEGS1 knockout cells. mRNA levels of FADS3 and DEGS1 were measured by quantitative real-time PCR in wild type (blue dots) and DEGS1 knockout cells (red dots). Values were normalized to GAPDH expression and calculated as fold change relative to wild type cells. Data are shown as median ± SD (error bars), n = 4, Mann-Whitney testing; ∗P < 0.05. B: Wild-type HAP1 and DEGS1 knockout cells were plated in 96-well cell culture plates and supplemented with increasing concentrations of SPB 18:1(14Z);O2 (0–10 μM). Cell viability was assessed after 48 h incubation time, luminescence was normalized to vehicle-treated cells. Data are shown as mean ± SD (error bars), n = 4.