TABLE 1.
Strains, plasmids, and phages used in this study
| Name | Genotype | Source |
|---|---|---|
| Strains | ||
| AMS150 | katF13::Tn10 | 38 |
| CAG18442 | thr-34::Tn10 | 57 |
| CAG18638 | zhh-21::Tn10 | 57 |
| DH5α | φ80dlacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17 (rK− mK+) supE44 thi-1 gyrA69 | Laboratory stock |
| PBL500 | lacZ::Tn5 lacIQ1 | 52 |
| PBL501 | PBL500; ΔdnaK52::Cmr | 52 |
| PBL502 | PBL500; rpoS-13::Tn10 | P1vir(AMS150) × PBL500 to tetracycline resistance |
| PBL503 | PBL500; ΔmutS-rpoS458 | A tetracycline-sensitive derivative of PBL502 |
| PBL504 | PBL501/pBN15 | This work |
| PBL505 | DH5α/pBN49 | This work |
| PBL603 | PBL500; rpoS13::Tn10 ΔdnaK52::Cmr | P1vir(pBN15/PBL501) × PBL502 to chloramphenicol resistance |
| PBL622 | PBL501; rpoH-1 | This work |
| PBL623 | PBL501; rpoH-2 | This work |
| PBL624 | PBL501; rpoH-3 | This work |
| PBL711 | PBL622/pBN15 | This work |
| PBL713 | PBL622; dnaK+ | This work |
| Plasmids | ||
| pBN15 | Ptac::dnaK+J+ lacI+ bla+ | 52 |
| pBN49 | dps+ | 8-kb BamHI-HindIII fragment from λ-205 subcloned into pACYC184 |
| pACYC184 | Tetr Cmr | Laboratory stock |
| pDS2 | Ptac::rpoH+ bla+ | 18 |
| Phages | ||
| P1vir | Laboratory stock | |
| λ-205 | 27 |