TABLE 2.
Characterization of the rpoH-1 mutant allele
| Strain (genotype) | GroEL inductiona | Growth at 43.5°Cb |
|---|---|---|
| PBL500 (wild type) | 10 | + |
| PBL501 (ΔdnaK52) | 2 | − |
| PBL622 (ΔdnaK52 rpoH-1) | 1 | − |
| PBL713 (rpoH-1) | 1 | − |
a
The values indicated represent the ratio of GroEL protein produced after 5 min of exposure to 42°C relative to the amount present in cells grown at 30°C prior to the imposition of heat. GroEL levels were determined by densitometry of autoradiograms of 35S-pulse-labeled proteins fractionated by SDS-PAGE.
b
Growth was determined by the ability of each strain to form single colonies on rich-medium plates. +, growth; −, no growth.