FIG. 4.

Effects of actin-modifying agents on the subcellular localization of MRTFs in the presence and absence of STARS. (A) NIH 3T3 cells transiently transfected with expression vectors encoding FLAG-tagged MRTFs with and without STARS were treated with LB (0.5 μM) or CD (1 μM) or cotransfected with vectors encoding C3 transferase (200 ng) or Rho19N (1 μg) in the absence of serum as indicated. The subcellular distribution of MRTFs was determined by immunostaining for the FLAG epitope. (B) The percentage of transfected cells from panel A that contained MRTFs in the nucleus in the presence of STARS was determined. At least 150 transfected cells were counted under each condition. Values are means ± standard deviations (error bars).