FIG. 5.

Effects of actin-modifying agents on the transcriptional activity of MRTFs in the presence and absence of STARS. NIH 3T3 cells were transiently transfected with expression vectors encoding STARS and MRTFs and SM22-luciferase or 4× CArG-luciferase as indicated. (A) Cells were treated with LB or CD as indicated. (B) Cells were cotransfected with expression vectors encoding C3 transferase (50 ng) or Rho19N (1 μg) as indicated. Values are expressed as the levels of activation of luciferase expression above the level with the reporter gene alone ± standard deviations (error bars).