FIG. 3.

Immunoblot analysis of B. bronchiseptica B013N containing fusion plasmids. Cells grown in iron (Fe)-replete (+) and iron-depleted (−) conditions were subjected to immunoblot analysis as described in Materials and Methods. The Alc-LacZ hybrid proteins were visualized by reactivity with anti-β-galactosidase monoclonal antibody. Lanes: β, β-galactosidase positive control; A, cells carrying alcA′-′lacZ plasmid pBB8; B, alcAB′-′lacZ (pBB15); C, alcABC′-′lacZ (pBB9); V, vector plasmid pBBR1MCS. The relative migration positions of protein standards (left) and estimated molecular masses of Alc-LacZ hybrid proteins (right) are indicated in kilodaltons.