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Basal media for ALI culture expansion (step 1)
| Reagent | Final concentration | Amount |
|---|---|---|
| PneumaCult-Ex Plus Basal Medium | N/A | 442.5 mL |
| PneumaCult-Ex Plus 50× Supplement | 1× | 50 mL |
| Hydrocortisone Stock Solution (200×) | 1× | 2.5 mL |
| Penicillin/Streptomycin (100×) | 1× | 5 mL |
| Total | N/A | 500 mL |
Store at 4°C for up to 2 months.
