2× MEM solution

Reagent	Final concentration	Amount
MEM media (10×)	2×	200 mL
GlutaMAX supplement (100×)	2×	20 mL
Sodium bicarbonate (7.5%)	0.24%	32 mL
HEPES (1 M)	20 mM	20 mL
Penicillin/Streptomycin (100×)	2×	20 mL
BSA solution (3.5%)	0.04%	12 mL
diH2O	N/A	696 mL
Total	N/A	1000 mL

Store at 4°C for up to 2 months.