FIGURE 1.

Homology modeling of canine Ly49 compared with murine Ly49.

(A) BLAST amino acid sequence comparisons for Ly49 between Mus musculus (top row) and Canis familiaris (bottom row) with the cysteine-to-tyrosine mutation regions (red) and the MHC-I interface region (blue) marked as shown. (B) Similarity between overlapping tertiary structures (murine in teal and canine in red) by AlphaFold and ColabFold are shown. Site of cysteine-to-tyrosine mutation is denoted by the black arrow. (C) In silico model for binding between murine and canine Ly49 (teal and red) with canine MHC-I (pink). Site of cysteine-to-tyrosine mutation is denoted by the black arrow, which is not predicted to be involved in the Ly49–MHC-I binding interface.