Fig. 2.

Plasma APOE protein levels are increased in APOE mice following 6 months on a Western diet. (A) A representative Western blot is shown of blood plasma from 12-month-old APOE2, APOE3 and APOE4 mice on a regular diet (RD) or a Western high-fat diet (WD; also marked by an asterisk) probed with the anti-human APOE monoclonal antibody HJ15.3. Western blot analysis of blood plasma from a wild-type mouse (right panel) is used as a negative control to show non-specific reactivity. The molecular weight of monomeric APOE protein is indicated, as are apparent higher molecular weight allele-dependent APOE homo- and/or heterdimers. (B) Quantification by sandwich ELISA showing APOE protein levels in blood plasma samples from APOE mice treated with regular diet (RD) or Western diet (WD). Data are expressed as the mean ± SEM. n(APOE2) = 7 RD, 5 WD; n(APOE3) = 7 RD, 8 WD; n(APOE4) = 5 RD, 11 WD.