Table 1.
Affinities for SPOP self-association and substrate binding
| SPOP variant (residues 28–359) | Isodesmic KDa [μM] | KD for peptide [μM]b | KD for cDAXX [μM]b |
|---|---|---|---|
| WT | 2.7 ± 0.4 | 9.8 ± 0.7 | 1.2 ± 0.1 |
| R221C | 14.8 ± 2.0 | 5.9 ± 0.4 | 3.2 ± 0.6 |
| R354H | 26.9 ± 2.5 | 5.1 ± 0.6 | 3.0 ± 0.4 |
| W131G | N.D. | No binding | N.D. |
| mutBACK | N.D. | 4.3 ± 0.3 | N.D. |
| mutBTB | N.D. | 5.7 ± 0.4 | N.D. |
| mutBTB-BACK | N.D. | 7.4 ± 0.4 | N.D. |
BACK, BTB and C-terminal kelch; BTB, broad-complex, tramtrack, and bric-a-brac; ND, not determined.
a
From CG-MAS; mean from at least three replicates ± S.D.
b
From fluorescence anisotropy assay; mean from at least three replicates ± S.D.