FIG. 3.

Nucleotide sequence of alcR. The nucleotide sequence (upper strand) of the 1,200-bp region of the B. pertussis 1.6-kb KpnI-PstI DNA fragment and the predicted amino acid sequence of AlcR in the one-letter code are shown. The transcription initiation sites (+1) determined by primer extension analysis using the designated antisense primer (arrow) are indicated. Sequences similar to the consensus Fur binding site (Fur Box) and the positions of the NgoAIV restriction endonuclease sites used to construct deletion mutants PM10 and BRM11 are shown.