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Localization of the iron-regulated PalcR promoter of alcR. RNA was isolated from B. pertussis wild-type strain UT25Sm1 (WT) and B. pertussis mutant PM-4, in which the alcA promoter-operator region is deleted, grown in iron-replete (+Fe) and iron-depleted (−Fe) conditions. Transcriptional initiation sites (+1) corresponding to two nucleotides, C and T (at positions 124 and 125, respectively), were mapped by primer extension analysis using the antisense primer shown in Fig. 3.
