TABLE 1.
Alcaligin siderophore activity of B. pertussis
| Strain | Siderophore activitya
|
|
|---|---|---|
| With iron | Without iron | |
| UT25Sm1 (wild type) | 0.018 ± 0.014 | 0.592 ± 0.014 |
| PM10/pP9KP (alcR+) | 0.000 ± 0.028 | 0.747 ± 0.006 |
| PM10/pRK415 (vector) | 0.000 ± 0.026 | 0.047 ± 0.005 |
Mean relative CAS siderophore activities [calculated as 1 − (Asample/ Areference), n = 3] of cultures of wild-type strain UT25Sm1 and alcR mutant PM10 carrying the indicated plasmids grown in iron-replete and iron-depleted media ± standard deviations. The reference value (Areference) was the absorbance value at 630 nm obtained with uninoculated medium.