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Mapping of the region of pEP279 conferring a Lac+ phenotype in the FURTA. Subclones of the 3.5-kb PstI insert were constructed by deletions in pEP279 using the following restriction enzymes: HII, HincII; K, KpnI; N, NarI; P, PstI; S, SmaI; SI, SacI; and SII, SacII. The recombinant plasmid-associated Lac phenotypes are indicated on the right. The deduced localization of the FBS is also shown (black box).
