TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant featuresa | Source or reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| H1717 | aroB fhuF::λplacMu; Apr | 54 |
| XL1-Blue | High-efficiency transformation; Tcr | Stratagene |
| SM10 | Mobilizing strain; Kmr | 50 |
| S17.1 | Mobilizing strain; Smr | 50 |
| B. avium 103004 | Smr | Institut Pasteur, Paris, France |
| B. bronchiseptica | ||
| BB1015 | Smr derivative of NL1015 but not rpsL | This study |
| BBEP205 | Derivative of BB1015 but alcR::Kmr | This study |
| B. parapertussis PEP | 37 | |
| B. pertussis | ||
| BPSM | Tohama I rpsL; Smr Nalr | 32 |
| BPEP184 | Derivative of BPSM but alcR::Kmr | This study |
| BP953 | Tohama I rpsL fhaB::lacZ ptx::phoA; Smr Nalr | 53 |
| BPEP214 | Derivative of BP953 but alcR::Kmr | This study |
| Plasmids | ||
| pBBR1MCS | Broad-host-range vector; Cmr | 29 |
| pBCSK+ | High-copy-number cloning vector; Cmr | Stratagene |
| pEP279 | pBCSK+ containing a 3.5-kb PstI fragment carrying the B. bronchiseptica alcR gene; Cmr | This study |
| pEP300 | Derivative of pEP279 with a Kmr cassette inserted in the alcR gene; Cmr Kmr | This study |
| pEP301 | pBBR1MCS carrying a 1.6-kb KpnI-PstI fragment derived from pEP279; Cmr | This study |
| pEP308 | Derivative of pJQ200KS+ carrying a Kmr cassette flanked by alcR-derived sequences; Gnr Kmr | This study |
| pEP319 | Derivative of pSS1129 carrying a Kmr cassette flanked by alcR-derived sequences; Gnr Apr Kmr | This study |
| pJQ200KS+ | Bordetella suicide vector; Gnr | 42 |
| pSS1129 | Bordetella suicide vector; Gnr Apr | 52 |
| pUC4K | Source of Kmr cassette | Pharmacia |
a
Apr, Cmr, Gnr, Kmr, Nalr, Smr, and Tcr, resistance to ampicillin, chloramphenicol, gentamicin, kanamycin, nalidixic acid, streptomycin, and tetracycline, respectively.