Table 4.
Log 16S rRNA gene copy numbers of specific bacterial groups for the different experimental conditions of modelled cecal microbiota F1, enumerated by qPCR
| Log10 16S rRNA gene copies of taxon/mL (mean ± SD) | |||||||
|---|---|---|---|---|---|---|---|
| Total bacteria | Firmicutes | Ruminococcaceae | Lactobacillus-Leuconostoc-Pediococcus spp. | Bacteroidetes | Enterobacteriaceae | Bifidobacteriaceae | |
| CR | |||||||
| Treatment | 10.3 ± 0.1 | 10.1 ± 0.1 | 10.1 ± 0.1 | 6.6 ± 0.4 | 9.1 ± 0.1 | 7.5 ± 0.2 | 7.7 ± 0.1 |
| 50G | |||||||
| Treatment | 10.3 ± 0.1 | 10.1 ± 0.0 | 10.1 ± 0.1 | 6.9 ± 0.3 | 9.2 ± 0.1 | 6.9 ± 0.1 | 7.4 ± 0.0 |
| Delta with CR | + 0.03 | + 0.07 | + 0.07 | + 0.3 | + 0.1 | - 0.6 | - 0.3 |
| P value with CR | 0.7776 | 0.2302 | 0.4216 | 0.3673 | 0.3586 | 0.0158 | 0.1161 |
| 100G | |||||||
| Treatment | 10.4 ± 0.1 | 10.1 ± 0.1 | 10.1 ± 0.1 | 7.2 ± 0.1 | 9.8 ± 0.2 | 7.8 ± 0.0 | 7.0 ± 0.5 |
| Delta with CR | + 0.2 | 0.0 | 0.0 | + 0.5 | + 0.7 | + 0.3 | -0.6 |
| P value with CR | 0.2061 | > 0.9999 | > 0.9999 | 0.1462 | 0.0031 | 0.1144 | 0.0859 |
Data are means ± SD for the last 3 days of each treatment period. Significant differences were calculated as compared to Control reactor (P < 0.05) by unpaired t-test. CR, Control reactor; 50G, 10 mM glycerol; 100G, 100 mM glycerol