Figure 4. PIT Enhances CD5 Expression on Resting CD4⁺ and CD8⁺ PBMCs.

(A and B) Box-and-whiskers plots representing changes in MFI of CD5 expression levels on unstimulated CD4⁺ (A) and CD8⁺ (B) pre- and post-PIT PBMCs from seven patients in treatment Group 2. Isotype control MFI values have been subtracted.

(C and D) CD5 levels on pre-PIT (heavy black line) and post-PIT (grey, filled) CD4⁺ and CD8⁺ PBMCs of one representative patient. M1 marks the CD5^low population, with a pre- to post-PIT decrease in CD5^lowCD4⁺ PBMCs from 6.6% (in black) to 1.5% (in grey), and a decrease in CD5^lowCD8⁺ PBMCs from 32.3% to 13.9%. M2 indicates the concomitant increases in CD5⁺CD4⁺ and CD5⁺CD8⁺ cells post-PIT. Changes in MFI values for the total pre- and post-PIT CD4⁺ or CD8⁺ populations of the single representative patient are shown in the upper right-hand corner of each histogram. The Wilcoxon signed rank test was used for statistical analysis.