Fig. 4.
Effect of NPC seeding on CO infection parameters. A Western blot of proteinase K (PK) digested PrP from the CJD infections using 3F4 anti-PrP antibody (uncropped blots are shown in Additional file 1: Fig. S6). Samples were PK treated with 10 µg/ml. Lane N is NBH + NPC. B Quantification of PrPRes western blot intensities. C RT-QuIC 50% seeding dose (SD50) for CJD samples with or without NPC (n = 6). D Example PrP histochemistry of the NPC unseeded and seeded organoids with SAF32 antibody. Purple arrows show deposits. Scale bar is 25 µm E IHC summary of abnormal PrP deposits. With number scoring positive over total number of organoids per group examined