FIG. 2.

Relative positions of insertions that define the apbE gene. The physical organization of insertions in the apbE gene at 49 min is represented. The amino acid sequence of ApbE adjacent to the MudJ insertion is compared to the amino acid sequence of the E. coli homolog. Primers used in PCR and/or sequencing analysis are indicated (small arrows). The positions of the Tn10d(Tc) insertions were determined by amplification with primer 1 or 3 and primers specific to the ends of the insertion elements. Primers 1 and 3 were used to amplify the gene from the chromosome for cloning and determination of its nucleotide sequence.