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FACS analysis of strains. DNA contents of exponentially growing cells of the wild-type L972 (h−) (A) and the sep1-1 leu1-32 h− (B), sep1-1 wee1-112 h− (C), and sep1-1 wee1-50 ade1 h− (D) mutants at 25°C and after shift-up to 35°C (E) were analyzed with a Becton Dickinson FACScan.
