FIG. 3.

Localization of actin in wild-type and sep1-1 wee1-112 h⁻ cells by immunofluorescence microscopy. (a and b) Exponentially growing wild-type cells stained with DAPI (a) and fluorescence-labeled antiactin antibody (b); (c and d) sep1-1 wee1-112 h⁻ cells stained with DAPI (c) and fluorescence-labeled antiactin antibody (d). The hyphae characteristic of sep1⁻ strains are broken because they were treated with a cell wall lytic enzyme to facilitate the uptake of antibodies. The bar represents 8 μm.