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Effect of delaying S phase on the formation of binucleate cells. Exponentially growing cultures were shifted from 25 to 36°C for 60 min and then returned to 25°C. The percentage of binucleate cells was determined at regular intervals in samples stained with DAPI. ⧫, wild-type L972 (h−); □, sep1-1 h− strain; ▿ sep1-1 cdc10 strain.
