Figure 2.

ZmTLA1 Transcripts Are Present in Unfertilized and Fertilized Egg Cells, in Microspores, and during Leaf Morphogenesis.

(A) Single cell RT-PCR with egg cells (EC), synergids (SY), central cells (CC), antipodal cells (AP), zygotes (Z) at different times after in vitro fertilization (IVF), differentiated mesophyll cells (MC), nucellus cells (NC), and washing buffer (Control).

(B) RNA gel blot hybridization (6 μg total RNA, 2-d exposure).

(C) RNA gel blot hybridization (10 μg total RNA, 21-d exposure). The size of the full-length ZmTLA1 transcript is between 300 and 500 nucleotides. 18S and 25S rRNA are shown as quantitative controls. HEC, high embryonic capacity; LEC, low embryonic capacity; Mi., microspore, Sc., scutellum.