Table 2.

Segregation Analysis of GPT1/gpt1-1 and GPT1/gpt1-2

Genotype	Kanr/PCR+	Kans/PCR−	Ratio	χ2 (P)
GPT1/gpt1-1	190	222	0.9:1	2.5 (P = 0.01)
GPT1/gpt1-2	176	217	0.8:1	4.2 (P = 0.01)

The inheritance of gpt1-1 was analyzed by counting kanamycin-resistant seedlings recovered from selfed GPT1/gpt1-1 plants. The inheritance of gpt1-2 was monitored using a PCR-based assay as described in Methods. The χ² test was used to compare the observed ratios with a predicted ratio of 1:1. Kan^r, kanamycin resistant; Kan^s, kanamycin sensitive.