Table 3.
Analysis of Genetic Transmission of gpt1-1 and gpt1-2
| Genotype (Female × Male) | Kanr/PCR+ | Kans/PCR− | TE (%) |
|---|---|---|---|
| GPT1/gpt1-1 × Ws-2 | 47 | 137 | 34 |
| Ws-2 × GPT1/gpt1-1 | 26 | 128 | 20 |
| GPT1/gpt1-2 × Ws-2 | 30 | 112 | 27 |
| Ws-2 × GPT1/gpt1-2 | 28 | 142 | 20 |
| GPT1/gpt1-2 × Col-0 | 13 | 36 | 36 |
| Col-0 × GPT1/gpt1-2 | 11 | 45 | 24 |
The inheritance of gpt1-1 was analyzed by counting kanamycin-resistant F1 progenies of the denoted crosses. The inheritance of gpt1-2 was monitored using a PCR-based assay as described in Methods. The TE was calculated according to Howden et al. (1998). Kanr, kanamycin resistant; Kans, kanamycin sensitive.