Figure 7.

Phenotypic Complementation by Introduction of the D11 Gene.

The entire D11 gene, excised at the restriction sites XbaI and EcoRI (Figure 4B), was subcloned into a binary vector, pPZP2H-lac, to generate pPZP2H-lac-D11. The pPZP2H-lac and pPZP2H-lac-D11 were introduced into the Agrobacterium, EHA101. Transformants were generated from d11-1 using the Agrobacterium-mediated transformation method. Transformant-D11 and transformant-control vector were obtained by the infections of Agrobacterium containing pPZP2H-lac-D11 and pPZP2H-lac as the control vector, respectively. T65 was the recurrent parent for d11-1 and used as the wild-type plant.

(A) Gross morphology. Left, the wild type (T65); center, transformant-D11; right, transformant-control vector. Arrows and arrowheads indicate mature leaves and ears, respectively. Bar = 20 cm.

(B) Seeds. See (A) for rice plants. Seed form of transformant-D11 was the same as that of the wild-type plant. Bar = 0.5 cm.