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. 2005 Mar;17(3):776–790. doi: 10.1105/tpc.104.024950

Figure 9.

Figure 9.

Figure 9.

Quantitative Analysis of Endogenous BR Intermediates in Wild-Type and d11 Plants.

BR intermediates were isolated from shoots (A) and florets before flowering (B) as described in Methods. Sterol and BR levels (ng/g fresh weight) of two d11 alleles and their respective recurrent parents are shown below each product. The levels in d11-1 and its recurrent parent, T65, are indicated right and left, respectively, in the top parts marked with open circles. The levels in d11-2 and its recurrent parent, Shiokari, are indicated right and left, respectively, in the bottom parts marked with closed squares. The biosynthetic steps, in which D11 protein may participate, are indicated as “possible defect step 1” in (A) and “possible defect step 2” in (B). The steps catalyzed by D2 and OsDWARF (BRD1) proteins are shown as D2 and OsDWARF, respectively. nd, not detected; na, not analyzed.